Journal: Journal of Nanobiotechnology
Article Title: ROS-Responsive MnO 2 nanozyme co-delivering cGAMP and TGF-β inhibitor synergistically activates STING signaling and remodels the immunosuppressive thyroid cancer microenvironment
doi: 10.1186/s12951-026-04346-w
Figure Lengend Snippet: Effects of cGAMP Combined with SB on Cell Proliferation, Apoptosis, and Related Signaling Pathway Protein Expression in TC Cells. ( A ) Schematic illustration of the experimental workflow showing the grouping of TPC-1 and BCPAP cells and subsequent detection procedures; ( B ) Western blot analysis of p-STING, p-TBK1, and p-IRF3 protein expression levels in TPC-1 and BCPAP cells; ( C ) Western blot analysis of p-SMAD2/3 protein expression levels in TPC-1 and BCPAP cells; ( D ) CCK-8 assay evaluating changes in the proliferative activity of TPC-1 and BCPAP cells; ( E ) Flow cytometric analysis of apoptosis levels in TPC-1 and BCPAP cells using Annexin V/PI staining; ( F ) Combination Index (CI) plots generated using the Chou-Talalay method, illustrating the relationship between the fraction affected (Fa) and CI values in BCPAP and TPC-1 cells (CI < 1 indicates synergism). All cell experiments were performed in triplicate. * indicates p < 0.05, ** p < 0.01, *** p < 0.001 between groups
Article Snippet: BCPAP and TPC-1 cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), while Nthy-ori 3 − 1 cells were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).
Techniques: Expressing, Western Blot, CCK-8 Assay, Activity Assay, Staining, Generated